Brain/Neural Tissue-on-Chips &

                         Hydrogel-based Bioassays/Biosensors

이수현, Lee, Soo Hyun

Principal Researcher

OFFICE

L7333A, +82-2-958-6755

LABS

L7 3rd floor

EDUCATION:

Dec 2008, Ph.D. University of Cincinnati, OH, USA, Electrical and Computer Engineering and Computer Science (BioMEMS)

Sep 2005, M.S. University of Cincinnati, OH, USA, Electrical and Computer Engineering and Computer Science (BioMEMS)

Feb 2002, B.S. Korea University, Seoul, Korea, Electrical Engineering

RESEARCH CAREER & ACTIVITIES
  • 03/2018- Present Principal Researcher, Center for BioMicrosystems, Korea Institute of Science and Technology (KIST)
  • 02/2010- Present, Senior Researcher, Center for BioMicrosystems, Korea Institute of Science and Technology (KIST)
  • 03/2012- Present, Associate Professor, Department of Biomedical Engineering, Korea University of Science and Technology (UST)
  • 04/2013-03/2014, Visiting Scholar, BioMiNT (Biomolecular Microsystems and Nano Tranducers) lab, University of Califonia, Irvine (Prof. Abraham P. Lee)
  • 10/2006-02/2010, Researcher, Center for BioMicrosystems, Korea Institute of Science and Technology (KIST)
  • 09/2002-09/2006, Graduate Research Assistant, Prof. Chong H. Ahn, Microsystems and Biomems Laboratory, University of Cincinnati, OH
RESEARCH INTERESTS
  • Ultra-high sensitive nano gap sensor for tau protein and PTM to diagnose Neurodegenerative disease such as AD and PD
  • Functional neural electrode for CNS and PNS
  • Implantable sensors such as IOP (intraocular pressure) sensor
  • Cell chip, Lab-on-a-chip for in-vitro blood-brain-barrier (BBB)
  • Piezoelectric resonator using PZT material in Nano scale
  • Polymer waveguide sensor and SPR (Surface Plasmon Resonance) device
  • RT (Reverse Transcription)-PCR Lab-on-a-chip using polymer materials
Postdocs

Yi Jae Lee

Senior research scientist

EDUCATION
Ph.D. Department of Electronic Engineering, Kwangwoon University (2013)
M.S. Department of Electronic Engineering, Kwangwoon University (2009)
B.S. Department of Electronic Engineering, Kwangwoon University (2007)

RESEARCH INTERESTS
  • BioMEMS, Electrochemical analysis, Biosensor, Bioelectronics

Hyungbeen Lee

Postdoctoral

EDUCATION
Ph.D. Biomedical Engineering, Yonsei University (2018)
B.S. Biomedical Engineering, Yonsei University (2012)

RESEARCH INTERESTS
  • Improvement of Atomic Force Microscopy (AFM) imaging and its applications
  • Development of a new nano/bio diagnostic platform based on a combination of Kelvin Probe Force Microscopy (KPFM) and nanoparticles
  • Development of a nano-mechanical biosensor by detecting the peptide cleavage
Researcher

Jae Young Park

Researcher

EDUCATION

M.S. Mechanical Engineering, Yonsei University (2018)
B.S. Mechanical Engineering, Yonsei University (2016)

RESEARCH INTERESTS
  • Ultra-high sensitive nano gap sensor for Alpha-synuclein to diagnose Neurodegenerative disease such as AD and PD
  • Two-dimensional material based Field-effect transistor and its biosensor applications

Jae Young Park

Researcher

EDUCATION

M.S. Electrical Engineering, Seoul National University (2018)
B.S. Electronics Engineering, Korea Aerospace University (2016)

RESEARCH INTERESTS

My research interests include point-of-care biosensors, bio-MEMS, bio-interfaces. Ultimately, I would like to pursue research using biosensor technology in the human body where it can be used for monitoring health, early detection of cancer and critical diseases. Elaborated biosensors and MEMS technology would be key in making such a detection method accessible.

Graduate Students

Hyewon Seo

Graduate Student

EDUCATION
B.S. Electronic Engineering, Korea Polytechnic University(2016)

RESEARCH INTERESTS
  • Ultra-high sensitive nano gap sensor for tau protein and PTM to diagnose Neurodegenerative disease
3D in vitro culture platform
Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network

In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate. Applying this method, we have reconstructed the CA3–CA1 hippocampal neural circuit three-dimensionally in a monolithic gel, in which CA3 neurons extend parallel axons to and synapse with CA1 neurons. Furthermore, we show that alignment of the fibrous scaffold facilitates the establishment of functional connectivity. This method can be applied for reconstructing other neural circuits or tissue units where anisotropic organization in a multi-modular structure is desired.

Hydrogel-based bioassay
Multiplexed detection of epigenetic markers using QD-encoded hydrogel microparticles

The array-based detection of modified histones, conventionally used for multiplexed analysis of epigenetic changes, requires pooling of samples from many subjects to analyze population-wise differences in the expression of histone markers and does not permit individualized analysis. We report multiplexed detection of genome-wide changes in various histone modifications at a single-residue resolution using quantum dot (QD)-encoded polyethylene glycol diacrylate (PEGDA) hydrogel microparticles.

Hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer's disease

Quantitative polymerase chain reaction (qPCR) renders profiling of genes of interest less time-consuming and cost-effective. Recently, multiplex profiling of miRNAs has enabled identifying or investigating predominant miRNAs for various diseases such as cancers and neurodegenerative diseases. Conventional multiplex qPCR technologies mostly use colorimetric measurements in solution phase, yet not only suffer from limited multi- plexing capacity but also require target-screening processes due to non-specific binding between targets and primers. We present hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer’s disease (AD). Our methodology promises two key advantages compared with the conventional so- lution-based PCR: 1) nearly no non-specific crosstalks between targets and primers, and 2) practically valuable multiplexing by spatial encoding within a single microchamber. Specifically, we immobilized hydrogel micro- posts (~ 400 μm in diameter) within commercially available polycarbonate PCR chips by multi-step ultraviolet (UV, 365 nm) exposure.

International Journal
  • Woongsun Choi, Sang Yun Yeoma, Junsun Kim, Seungwon Jung, Seungho Jung, Tae Soup Shim, Sang Kyung Kim, Ji Yoon Kang, Soo Hyun Lee, Il-Joo Cho, Jungkyu Choi, Nakwon Choi, “Hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer’s disease” Biosensors and Bioelectronics, (2018) 235-244
  • Han-Jun Kim, Dong Nyoung Heo, Yi Jae Lee, Sang Jin Lee, Ji Yoon Kang, Soo Hyun Lee*, II Keun Kwon & Sun Hee Do, “Biological assessments of multifunctional hydrogel-decorated implantable neural cuff electrode for clinical neurology application” Scientific Reports, 20171110
  • Heo, D. N., H.-J. Kim, Y. J. Lee, M. Heo, S. J. Lee, D. Lee, S. H. Do, H. Lee*, I. K. Kwon* (2017). “Flexible and highly biocompatible nanofiber-based electrodes for neural surface interfacing.” ACS NANO: 201703 (JCR Ranking: 3.082%, IF: 13.334)
  • Lee, Y. J., H.-J. Kim, J. Y. Kang, S. H. Do and H. Lee* (2017). “Biofunctionalization of Nerve Interface via Biocompatible Polymer-Roughened Pt Black on Cuff Electrode for Chronic Recording.” Advanced Healthcare Materials: 1601022, 201703
  • Hwang, D. G., Y. M. Chae, N. Choi, I.-J. Cho, J. Y. Kang and H. Lee* (2016). “Label-free detection of prostate specific antigen (PSA) using a bridge-shaped PZT resonator.” Microsystem Technologies: 1-8.
  • Lee, Y. J., H.-J. Kim, S. H. Do, J. Y. Kang and H. Lee* (2016). “Characterization of nerve-cuff electrode interface for biocompatible and chronic stimulating application.” Sensors and Actuators B: Chemical 237: 924-934.
  • Ji, J.-h., K.-s. Shin, S. Kang, H. Lee, J. Y. Kang, S. Kim and S. C. Jun (2016). “Fundamental monomeric biomaterial diagnostics by radio frequency signal analysis.” Biosensors and Bioelectronics 82: 255-261.
  • Heo, D. N., S.-J. Song, H.-J. Kim, Y. J. Lee, W.-K. Ko, S. J. Lee, D. Lee, S. J. Park, L. G. Zhang J. Y. Kang, S. H. Do, H. Lee*, I. K. Kwon* (2016). “Multifunctional hydrogel coatings on the surface of neural cuff electrode for improving electrode-nerve tissue interfaces.” Acta Biomaterialia.
  • Jang, C.-I., K.-S. Shin, M. J. Kim, K.-S. Yun, K. H. Park, J. Y. Kang and H. Lee* (2016). “Effects of inner materials on the sensitivity and phase depth of wireless inductive pressure sensors for monitoring intraocular pressure.” Applied Physics Letters 108(10): 103701.
  • Lee, Y. J., S. J. Park, K.-S. Yun, J. Y. Kang and H. Lee* (2016). “Enzymeless glucose sensor integrated with chronically implantable nerve cuff electrode for in-situ inflammation monitoring.” Sensors and Actuators B: Chemical 222: 425-432.
  • Kim, Y. W., M. J. Kim, K. H. Park, J. W. Jeoung, S. H. Kim, C. I. Jang, H. Lee, J. H. Kim, S. Lee and J. Y. Kang (2015). “Preliminary study on implantable inductive‐type sensor for continuous monitoring of intraocular pressure.” Clinical & experimental ophthalmology 43(9): 830-837.
  • Shin, K.-S., C.-I. Jang, M. J. Kim, K.-S. Yun, K. H. Park, J. Y. Kang and H. Lee* (2015). “Development of Novel Implantable Intraocular Pressure Sensors to Enhance the Performance in in vivo Tests.” Journal of Microelectromechanical Systems 24(6): 1896-1905.
  • Park, S. J., Y. J. Lee, D. N. Heo, I. K. Kwon, K.-S. Yun, J. Y. Kang and H. Lee* (2015). “Functional nerve cuff electrode with controllable anti-inflammatory drug loading and release by biodegradable nanofibers and hydrogel deposition.” Sensors and Actuators B: Chemical 215: 133-141.
  • Chae, M.-S., S.-M. Lee, Y. K. Yoo, H. Lee, J. Kim, T. S. Kim, D. J. Ahn, J. H. Lee and K. S. Hwang (2014). “Fabrication and characterization of piezoelectric driven microdiaphragm resonating sensor for a biosensing application.” Journal of Electroceramics 32(4): 383-389.
  • Hwang, H., B. Kang, H. Lee, C.-I. Jang, J. Y. Kang and C. Park (2014). “Resonance frequency effects of an external coil for IOP sensor applications.” Journal of Electromagnetic Waves and Applications 28(7): 790-801.
  • Chu, J.-U., K.-I. Song, A. Shon, S. Han, H. Lee, J. Y. Kang, D. Hwang, J.-K. F. Suh, K. Choi and I. Youn (2013). “Feedback control of electrode offset voltage during functional electrical stimulation.” Journal of neuroscience methods 218(1): 55-71.
  • Chu, J.-U., K.-I. Song, S. Han, H. Lee, J. Y. Kang, D. Hwang, J.-K. F. Suh, K. Choi and I. Youn (2013). “Gait phase detection from sciatic nerve recordings in functional electrical stimulation systems for foot drop correction.” Physiological measurement 34(5): 541.
  • Kang, B., H. Hwang, H. Lee, J. Y. Kang, J.-H. Park, C. Seo and C. Park (2013). “A wireless intraocular pressure sensor with variable inductance using a ferrite material.” JSTS: Journal of Semiconductor Technology and Science 13(4): 355-360.
  • Hwang, D. G., Y. M. Chae, K. S. Hwang, J. Y. Kang and H. Lee* (2012). “Fabrication and characterization of PZT (lead zirconate titanate) bridge-shaped resonator for mass sensing application.” Journal of electroceramics 29(3): 225-234.
  • Kim, C., J. H. Bang, Y. E. Kim, H. Lee and J. Y. Kang (2012). “On-chip anticancer drug test of regular tumor spheroids formed in microwells by a distributive microchannel network.” Lab on a chip 12(20): 4135-4142.
  • Chu, J.-U., K.-I. Song, S. Han, H. Lee, J. Kim, J. Y. Kang, D. Hwang, J.-K. F. Suh, K. Choi and I. Youn (2012). “Improvement of signal-to-interference ratio and signal-to-noise ratio in nerve cuff electrode systems.” Physiological measurement 33(6): 943.
  • Kim, C., K. S. Lee, J. H. Bang, Y. E. Kim, M.-C. Kim, K. W. Oh, H. Lee and J. Y. Kang (2011). “3-Dimensional cell culture for on-chip differentiation of stem cells in embryoid body.” Lab on a Chip 11(5): 874-882.
  • Kim, C., S. Chung, Y. E. Kim, K. S. Lee, H. Lee, K. W. Oh and J. Y. Kang (2011). “Generation of core-shell microcapsules with three-dimensional focusing device for efficient formation of cell spheroid.” Lab on a Chip 11(2): 246-252.
  • Lee, S. H*., J. H. Jung, Y. M. Chae, J.-K. F. Suh and J. Y. Kang (2010). “Fabrication and characterization of implantable and flexible nerve cuff electrodes with Pt, Ir and IrOx films deposited by RF sputtering.” Journal of Micromechanics and Microengineering 20(3): 035015.
  • Su-Kyoung Chae, Chang-Ha Lee, Soo Hyun Lee, Tae-Song Kim and Ji Yoon Kang, “Oil droplet generation in PDMS microchannel using an amphiphilic continuous phase”, Lab Chip, 2009, 9, 1957–1961
  • Choong Kim, Kang Sun Lee, Young Eun Kim, Kyu-Jung Lee, Soo Hyun Lee, Tae Song Kim and Ji Yoon Kang, “Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability”, Lab Chip, 2009, 9, 1294–1297
  • Soo Hyun Lee*, Sung-Woo Kim, Ji Yoon Kang and Chong H. Ahn, “A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics”, Lab Chip, 2008, 8, 2121–2127
  • Zou, S. Lee, and C.H. Ahn, “A Polymer Microfluidic Chip with Interdigitated Electrodes Arrays for Simultaneous Dielectrophoretic Manipulation and Impedimetric Detection of Microparticles”, IEEE Sensors Journal, VOL. 8, 527-534, (2008)
  • Chunyan Li, Soohyun Lee, Andrew Gorton, Mark J. Schulz and Chong H. Ahn, “Flexible Dome and Bump Shape Piezoelectric Tactile Sensors using PVDF-TrFE Copolymer”, Journal of Microelectromechanical Systems, VOL. 17, 334-340, (2008)
    (*: first and corresponding author)
Planned Baseball Outing
January 2018: Lab Opening
Information

We are a team dedicated acheiving the best in brain science research while providing an ideal environment for innovative thinking and career building skills. We are currently seeking exceptional candidates who share our passion to join our team. If you are interested in joining our Lab, please email changhyuk@kist.re.kr

Research Keywords