Brain/Neural Tissue-on-Chips &

                         Hydrogel-based Bioassays/Biosensors

최낙원, Nakwon Choi

Principal Researcher

OFFICE

L7325A

LABS

L7336, L7226

E-mail

nakwon.choi(at)kist.re.kr

Phone

+82 2-958-6742

EDUCATION:

Cornell University, Ithaca, NY, USA
School of Chemical and Biomolecular Engineering
Ph.D. in Chemical Engineering (Feb. 2010)
M.S. in Chemical Engineering (Mar. 2008)

Seoul National University, Seoul, Korea
School of Chemical and Biological Engineering
B.S. cum laude in Chemical Engineering (Mar. 1997 – Feb. 2004)

RESEARCH CAREER & ACTIVITIES

Mar. 2017 – Present

  • Principal Researcher, Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST)

Jan. 2012 – Feb. 2017

  • Senior Researcher, Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST)

Jun. 2010 – Dec. 2011

  • Presidential Postdoctoral Fellow, Hit Discovery Group, Center for Proteomic Chemistry, Lead Finding Platform, Novartis Institutes for Biomedical Research (NIBR), Cambridge, MA, USA
  • Research Affiliate (Postdoctoral Fellow), Department of Chemical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA

Jan. 2010 – May 2010

  • Postdoctoral Associate, School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA

Aug. 2004 – Dec. 2009

  • Research Assistant/Teaching Assistant, School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA
RESEARCH INTERESTS
  • Integrated Neural Interface
    – Optoelectronic Neural Interface
    • Lens-free Implantable Fluorescence Imaging Device
    • Custom CIS (SPAD, APD) IC for Light-field Imaging
    • μLED and SPAD array on CMOS for in-vivo Fluorescence Imaging on Awake or Freely
  • Behaving Animal
    • Low-power Wireless Data and Power Connectivity for IMD
    – Mixed signal IC design for Low Power IMD
Postdocs

We are Looking For Prospective Postdocs

Postdoctoral Researcher
Graduate Students

Woongsun Choi

Graduate student

EDUCATION
B.S.Chemical & Biological Engineering, Korea University (2015)

RESEARCH INTERESTS
  • Hydrogel-based bioassay

Sohyeon Jeong

Graduate Student

EDUCATION
B.S. Food Science and Technology, Seoul National University of Science and Technology (2015)

RESEARCH INTERESTS
  • 3D in vitro culture platform for reconstructtion of neural circuit

Junbeom Kim

Graduate Student

EDUCATION
B.S. Chemical & Biological Engineering, Korea University (2016)

RESEARCH INTERESTS
  • Hydrogel-based bioassay

Hyunwook Kang

Graduate Student

EDUCATION
B.S. Mechanical Engineering, Korea University (2018)

RESEARCH INTERESTS
  • 3D in vitro culture platforms

Svitalana Belaya

Graduate Student

EDUCATION
B.S. Biological faculty, Belarusian State University (2017)

RESEARCH INTERESTS
  • Hydrogel-based bioassay
Alumni

Sangyun Yeom

Alumini

EDUCATION

B.S. Chemical & Biological Engineering, Korea University (2014)

RESEARCH INTERESTS
  • Hydrogel-based bioassay

Brittany Sincox

Alumini

EDUCATION

M.Res./Ph.D., Neuroscience, University College London and National Institutes of Health

Undergraduate, Biochemistry, Grand Valley State University

RESEARCH INTERESTS
  • Neuropharmacology
3D in vitro culture platform
Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network

In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate. Applying this method, we have reconstructed the CA3–CA1 hippocampal neural circuit three-dimensionally in a monolithic gel, in which CA3 neurons extend parallel axons to and synapse with CA1 neurons. Furthermore, we show that alignment of the fibrous scaffold facilitates the establishment of functional connectivity. This method can be applied for reconstructing other neural circuits or tissue units where anisotropic organization in a multi-modular structure is desired.

Hydrogel-based bioassay
Multiplexed detection of epigenetic markers using QD-encoded hydrogel microparticles

The array-based detection of modified histones, conventionally used for multiplexed analysis of epigenetic changes, requires pooling of samples from many subjects to analyze population-wise differences in the expression of histone markers and does not permit individualized analysis. We report multiplexed detection of genome-wide changes in various histone modifications at a single-residue resolution using quantum dot (QD)-encoded polyethylene glycol diacrylate (PEGDA) hydrogel microparticles.

Hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer's disease

Quantitative polymerase chain reaction (qPCR) renders profiling of genes of interest less time-consuming and cost-effective. Recently, multiplex profiling of miRNAs has enabled identifying or investigating predominant miRNAs for various diseases such as cancers and neurodegenerative diseases. Conventional multiplex qPCR technologies mostly use colorimetric measurements in solution phase, yet not only suffer from limited multi- plexing capacity but also require target-screening processes due to non-specific binding between targets and primers. We present hydrogel micropost-based qPCR for multiplex detection of miRNAs associated with Alzheimer’s disease (AD). Our methodology promises two key advantages compared with the conventional so- lution-based PCR: 1) nearly no non-specific crosstalks between targets and primers, and 2) practically valuable multiplexing by spatial encoding within a single microchamber. Specifically, we immobilized hydrogel micro- posts (~ 400 μm in diameter) within commercially available polycarbonate PCR chips by multi-step ultraviolet (UV, 365 nm) exposure.

International Journal
Planned Baseball Outing
January 2018: Lab Opening
Information

We are a team dedicated acheiving the best in brain science research while providing an ideal environment for innovative thinking and career building skills. We are currently seeking exceptional candidates who share our passion to join our team. If you are interested in joining our Lab, please email changhyuk@kist.re.kr

Research Keywords